UV- visible spectrophotometer
UV spectroscopy is an important tool in analytical chemistry. The other name of UV (Ultra-Violet) spectroscopy is Electronic spectroscopy as it involves the promotion of the electrons from the ground state to the higher energy or excited state. It measures the intensity of light passing through a sample (I), and compares it to the intensity of light before it passes through the sample (Io). The ratio I / Iois called the “transmittance”, and is usually expressed as a percentage (%T).UV/Vis spectroscopy is routinely used in analytical chemistry for the quantitative determination of different analytes, such as transition metal ions, highly conjugated organic compounds, and biological macromolecules. Determination is usually carried out in solutions.