The Faculty of Life Science has organized two days National On-Hand workshop entitled “Microbial Biotechnology Approach for Profiling Industrially Important Fungi”

Department: Life Sciences

Year/Semester: Students of B.Sc. Final Year and M.Sc. from different branches of Life Sciences of different colleges.

Participant: 94

Activity: Two Days National Hands-on Workshop .
Date and Duration: 9-10 March, 2021. 9.30 AM  to 4:30 PM
Title:  Microbial Biotechnology Approach for Profiling Industrially Important Fungi.
Resource Person: Dr. R.K Garg, Joint project Director and Head, Centre of Excellence in Biotechnology,  M.P. Council of Science and technology (MPCST), Bhopal

Brief description/ Remarks: 

Faculty of Life Sciences Mandsaur University has organized two days National On-Hand workshop entitled “Microbial Biotechnology Approach for Profiling Industrially Important Fungi” for the students of B.Sc. Final Year and M.Sc. of different branches of Life Sciences on 9-10 March, 2021. The workshop emphasized on delivering hands-on performance of modules related to the topic and demonstration on different sophisticated instruments of the department. Overall, the workshop has been divided into five different modules for two days.

The workshop started with the inauguration program which was chaired by the chief guest Dr. R.K Garg Project Director, MPCST, Bhopal with Dr. Shailendra Sharma Vice chancellor Mandsaur University, Dr. Amit Jain Coordinator IQAC Mandsaur University and Dr. Swapnil Goel Dean  Research & deveopment Mandsaur University. In the inaugural event, Dr. Shekhar Jain, HOD Faculty of Life Sciences delivered welcome address and briefed all participants about the workshop, facilities available in the department and achievements of the students. He has also given a brief introduction about the chief guest of the event. Further, honorable Vice chancellor of Mandsaur university praises the participants and briefed the research activities and schemes running by the university for the research based development of the students. Dr. R.K Garg Chief guest of the event key addressed the participants. He discussed about various activities and schemes of MPCST for the students, faculties and institutions in details. He also emphasized on the bright prospects of Microbiology and Biotechnology and different government funding schemes for start-up in this sector with innovative idea. Vice Chancellor Dr. Shailendra Sharma honoured the Chief guest with a Memento and certificate of Recognition. The Inaugural ceremony winds up with vote of thanks delivered by Dr. Taniya Sengupta Rathore, Associate Professor Faculty of Life Sciences, Mandsaur University.

Module 1: Screening of Amylase producing Fungi– Dr. Ganshyam Prajapat, Assistant Professor Faculty of Life Sciences has briefed participants about overview of industrially important fungi and carried hands-on session along with Prof. Teena Yadav, Assistant Professor, Faculty of Life Sciences. In this module they focused on screening of fungi with efficacy to produce amylase enzyme having industrially importance.

Module 2: Isolation of genomic DNA from fungi and Separation by Agarose gel electrophoresis-The session was taken by Dr. Taniya Sengupta Rathore and Dr. Khushdil Khan wherein genomic DNA isolated from screened fungus and separated using Agarose gel electrophoresis followed by the visualization of DNA bands in UV transilluminator.

Module 3: PCR amplification of fungus gene by using universal primer– Dr. Shekhar Jain has briefed the participants about the principle, mechanism and job prospects of PCR technique. Successively, the PCR amplification of fungi gene by using universal and specific primer was performed and demonstrated by him along with Prof. Yogendra Verma, Assistant Professor to the participants.

Module 4: Identification of fungus using sequence alignment tools– Online sequence alignment of the PCR product for the identification of unknown fungus was demonstrated by Dr. Ashish Warghane and Dr. Ganshyam Prajapat using Bioinformatics tool BLAST at computer lab of the department.

Module 5: Demonstration of various advance instruments available in the department was given expert faculty members to the participants. During demonstration each expert has explained the working principle, mechanism, protocol and applications of the specific instrument.

·   Camera attached Binocular Light Microscope : Prof. Teena Yadav
·   UV Spectrophotometer &  Cooling Centrifuge : Dr. Taniya Sengupta Rathore
·   SDS PAGE : Prof. Yogendra Verma
·   Plant tissue culture Lab : Dr. Khusdil Kha
·   Fermentor : Prof. Heena Shah
·   ELISA Reader : Dr. Ashish Warghane
·   Microtome : Prof. Meenakshi Jain

 

The workshop was concluded by the Dr. Shekhar Jain by enlightening the features and achievements of the department. He also discussed about the career and future prospects of the ongoing subjects. He gave vote of thank to all participants, students of life sciences and organizing committee members for completing the event successfully. The engagement of all the participants appreciated by providing them with the participation certificates.

All the faculty members of the department and students have actively contributed for making it a successful event.

Photos:

Outcome:  All the participants have actively participated in the workshop. They learnt the practical approach of various advance techniques related to Biotechnology and Microbiology. They also learnt how basic research and advance tools use to identify industrially important fungi. For most of the participants, it was first time they were seeing a real PCR one of the most advance tool in working condition. This workshop helps the student to pursue carrier in the field of Research and development. Such type of workshop helps university to grow and promote at national level. Positive feedbacks were received from all participants by filling feedback form. Students also have shown desire to participate in such type of upcoming activities.

No. of participants: 94 participants from different colleges.

Prepared by: Dr. Taniya Sengupta Rathore, Associate Professor, Faculty of Life Science, Mandsaur University

Verified and Submitted by (HOD/HOI): Dr. Shekhar Jain