Sophisticated Instrumentation Facility, Faculty of Pharmacy

Mandsaur University, Mandsaur

HPLC (High Performance Liquid Chromatography)

Make and Model

Waters, USA and Gradient pump delta600 & detector PDA2998

Specifications

Number of solvents One to four
Solvent conditioning Vacuum degas, two (2) operating modes, four (4) chambers, ≈<500 uL internal volume per chamber> <500uL interval volume per chamber
Programmable flow rate range 0.000 and 0.010 to 10.000 mL/min in 0.001-mL/min. increments
Typical operating flow rate range 0.050 to 5.000 mL/min in 0.001-mL/min increments
Gradient profiles Eleven (11) gradient curves [including linear, step (2 curves), concave (4 curves), and convex (4 curves)]
Flow ramping Time (0.01 to 30.00 min in 0.01-min increments) to reach maximum flow rate
Maximum operating pressure 5000 psi (345 bar) (0.010 to 3.000 mL/min) Programmable upper and lower limits. Pressure fall-off at >3.000 mL/min.
Pressure ripple ≤2.5% (1 mL/min, degassed methanol, at 1700 psi backpressure)

Applications

Separation of different component of drugs/ synthetic mixture/trace organic/pesticide and PAHs etc.

HPTLC (High Performance Thin Layer Chromatography)

Make and Model

CAMAG, Switzerland

Specifications

Components available are: Linomat 5 for sample application, AMD2 for automated multiple development, twin trough tanks of different sizes for manual single solvent developments, HPTLC various system for method development, TLC Scanner 3 for detection, TLC/HPTLC documentation system with digital camera, Reprostar TLC/HPTLC viewing box with UV system, TLC plate heater, TLC plate coater, dip tanks, dedicated computer with winCATS Planar Chromatography System Manager.

Applications

  • Chromatography” is visible and simple
  • Little or no sample preparation
  • Analysis of dozens of samples, concurrently
  • Upto 8 detection modes, without repeating chromatography
  • HPTLC Fingerprint- chemical composition of mixtures, represented as photographic images
  • Very low cost of analysis and maintenance

FTIR (Fourier-Transform Infrared Spectroscopy)

Make and Model

Shimadzu Japan and FTIR-8400S

Specifications

  • Optical system- Single beam optics
  • Detector -Temperature controlled high sensitivity detector (DLATGS detector)
  • S/N ratio -Greater than 20,000:1 (KRS-5 window, 4cm-1, 1 minute, 2200cm-1, P-P)
  • Wavenumber range- 7,800cm-1 – 350cm-1
  • Sample compartment- W200mm x D230mm x H170mm

Applications

Basic drug research and structural elucidation, Formulation development and validation, Quality control processes for incoming and outgoing materials, Packaging testing

UV-Visible Spectrophotometer

Make and Model

Shimadzu Japan and UV-1900i

Specifications

  • Optical System: Double Beam Optics Wavelength Range: 190 to 1,100 nm
  • Spectral bandwidth: 1 nm (190 to 1,100 nm)
  • Photometric Range: Absorbance -4 to 4 Abs Transmittance – 0% to 400%
  • Source: Halogen (WI) and Deuterium Lamp.
  • Detector: Silicon Photodiode Supplied with below accessories:
  • Shimadzu Lab Solutions UV-Vis Software

Applications

Measurement/study in the region including functional group Metal complexes, Kinetic study, different element transition, Specific bond such as D- orbital participation

Differential Scanning Calorimetry

Make and Model

Shimadzu, Japan and DSC-60

Specifications

Principle Heat Flux Type
Heat Flow Range ± 40µW
Temperature Range -150 to 600° C
Atmosphere Inert gas or air
Power supply 100/120 VAC 800AV

Applications

Thermal analysis study including transition temperature/exothermic heat flow measurement, including melting point, crystallization behaviour, phase transition and thermal stabilization etc.

AAS (Atomic Absorption Spectroscopy)

Make and Model

SHIMADZU, Japan and AA-7000

Specifications

The AA-7000 series of Atomic Absorption Spectrophotometers features high-sensitivity analysis, flexible system configuration, and a compact footprint for user-friendly operation. In addition, the AA-7000 systems are the first AAs to employ a vibration sensor as standard. The AA-7000 series ensures efficient and comfortable operation in your laboratory.

Applications

Trace element analysis study in rock, oil plants and biological sample etc

Accelerated Solvent Extractor

Make and Model

Thermo Scientific, USA and ASE150

Specifications

  • Elevated temperature (up to 200°C) and pressure (1500 psi) reduce extraction times and solvent consumption
  • Fast pump (70mL/min.) enables fast extractions (typically 12 to 20 mins.)
  • Dramatically reduced solvent consumption

Applications

Using organic acids and aqueous solvents or acids and bases at high temperatures and pressures, the Thermo Scientific Dionex ASE Accelerated Solvent Extraction system extracts compounds from solid and semi-solid samples quickly with small solvent volume. You will save time, solvent, and money, and generate results in a fraction of the time compared to traditional techniques such as Soxhlet or sonication.

Gas Chromatograph

Make and Model

Thermo scientific, USA and Trace1110GC

Specifications

Performance Specifications

  • Typical Retention Time Repeatability: 0.008 min
  • Column Oven (H × W × D): 30.9 x 29.0 x 26.0 cm; 22.6 L
  • Pressure Range: 0–1000 kPa (0–145 psi)
  • 4 Detectors Available: Flame Ionization Detector, Thermal Conductivity Detector, Electron Capture Detector, Flame Photometric Detector and Nitrogen Phosphorus Detector Acquisition Rate: Up to 50 Hz Pneumatics: Manual pneumatics or EPC for gas flow control

Applications

Determination of Organic Volatile Impurities by GC, quality of products in the chemical industry; or measuring toxic substances in soil, air or water

Tablet Dissolution Tester

Make and Model

Electro lab, India and TDT-08L

Specifications

Speed range: Variable speed from 20 to 250 RPM
• Speed accuracy: +0.5 RPM
• Temperature range: 20o C to 40o C
• Temperature accuracy: +0.1o C
• Printer interface: RS 232 Connectivity
Applications

Tablet Dissolution Test Apparatus – 912 is an instrument for the determination of active drug ingredient in any pharmaceutical formulation – tablet or capsule as per USP, BP and IP specifications. This apparatus evaluates adequate bio-availability and provides necessary information to formulate in the development of therapeutically optimum dosage forms. This dissolution testing device plays an important role in product development, quality control and research.

Karl Fischer Apparatus

Make and Model

Analab, India and µAquaCal50

Specifications

  • Method of Detection : Conductometric
  • Backlit LCD Display : 20 x 4 Line Alphanumeric
  • Range of Moisture Detection : less than 20 PPM to 100%
  • Electrode: Dual Platinum Electrode.
  • End Point Sensitivity of Moisture Detection : Users Adjustable

Applications

Karl Fischer Titration is a technique for the determination of moisture content. Karl Fischer provides for the specific detection of water content in a product.

PLETHYSMOMETER

Make: Panlab, Harvard apparatus, Spain

Model: LE7500

Details:

The Digital Water Plethysmometer is designed to provide a highly useful tool in the measurement of small volume changes. This test is typically used to follow the evolution of the inflammatory response experimentally induced in rodents and to screen potential antiinflammatory or anti-edema properties of pharmacological substances.

Application: Anti-inflammatory drug screening, Anti-oedema drug screening, Inflammation, Post-operative pain

 

RANDALL & SELITTO APPARTUS (RAT PAW PRESSURE INSTRUMENT)

Make:  Panlab, Harvard apparatus, Spain

Model:  LE7306

Details:

The Panlab LE7306 paw & test pressure meter is used for performing the Randall & Selitto test in rodents. This test is based on determination of the animal threshold response to pain induced by the application of a uniformly increasing pressure on the paws or the tail.

Application: Pain disorders, Hyperanalgesics, Neuropathy, Inflammation, Post-operative pain, Analgesics screening.

 

Non-Invasive Blood Pressure Meter (NIBP)

Make:  Panlab, Harvard apparatus, Spain

Model:  LE5002

Details:

Panlab NIPB system provides an easy and reliable technique to measure systemic blood pressure and cardiovascular parameters in rodents without any invasive catheterization. The system is composed of a NIBP control unit, pressure cuff & pulse transducer, a heater, restrainers and optional software options. Different models of these components are available depending on the experiment request.

Applications:  Cardiovascular Disorder, Heart Rate, Blood Pressure, Pulse Rate.