A centrifuge is a device for separating particles from a solution according to their size, shape, density viscosity of the medium and rotor speed. Refrigerated centrifuge works on the concept of sedimentation principle by holding up the sample tubes with a capacity of 2ml, 10ml and 50ml in rotation around a fixed axis. In this, the centripetal force causes the denser substances to separate out along the radial direction in the bottom of the centrifuge tube. The rate of the centrifugation is calculated by the acceleration applied to the sample and it is typically measured in revolution per minute (RPM) or relative centrifugal force (RCF). This equipment is extensively used in chemistry, biology, and biochemistry for isolating and separating suspensions. It additionally provides the cooling mechanism to maintain the uniform temperature throughout the operation of the sample.